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Application note 32: Cropscan 2000B, Preliminary Evaluation of Protein Analysis in Sorghum.
|
Sample |
Predicted Y |
Measured Y |
Difference |
|
|
|
|
|
|
|
RT007 |
6.56 |
6.59 |
0.03 |
|
|
|
|
|
|
|
30208252 |
7.28 |
7.17 |
-0.11 |
|
|
|
|
|
|
|
10 |
8.12 |
7.88 |
-0.24 |
|
|
|
|
|
|
|
30208021 |
8.49 |
8.44 |
-0.05 |
|
|
|
|
|
|
|
2 |
8.71 |
8.78 |
0.07 |
|
|
|
|
|
|
|
H2399 |
9.04 |
9.39 |
0.35 |
|
|
|
|
|
|
|
4 |
9.99 |
9.94 |
-0.05 |
|
|
|
|
|
|
|
30208028 |
10.35 |
10.67 |
0.32 |
|
|
|
|
|
|
|
H2402 |
11.01 |
11.08 |
0.07 |
|
|
|
|
|
|
|
110829 |
11.68 |
11.57 |
-0.11 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Bias |
0.03 |
|
|
|
|
|
|
|
|
|
Std Dev |
0.19 |
|
|
|
|
|
Procedure: Approximately 50 sorghum samples scanned on Cropscan 2000B in an 18mm pathlength cell, 5 replicates per sample.
Sorghum protein determined by Bunge Feedstock laboratories by Kjeldahl analysis.
Preliminary calibration developed on 10 samples covering a wide range of protein content. Partial least squares regression was the method used and the calibration statistics are shown in the results section for the average of the 5 replicates.
Results:
Conclusion: The above results show that the Cropscan 2000B whole grain analyser can be calibrated to measure the protein content of sorghum samples, which come from a wide variety of types and seasons. Slight adjustments to the pathlength and more data should lead to a robust calibration. Moisture analysis is already known to be successful in sorghum with prediction statistics of about 0.2%.
Brad Swarbrick
Applications Chemist
29/11/2001